400.000 YEAR OLD HUMAN DNA

400,000-year-old human DNA adds new tangle to our origin story

Alan Boyle, Science Editor         NBC News

18 hours ago

Image: Sima de los Huesos people

Javier Trueba / Madrid Scientific Films
The Sima de los Huesos people lived about 400,000 years ago in  Spain.

The oldest human DNA ever recovered is throwing scientists for a loop: The 400,000-year-old genetic material comes from bones that have been linked to Neanderthals in Spain — but its signature is most similar to that of a different ancient human population from Siberia, known as the Denisovans.

The researchers who did the analysis said their findings show an “unexpected link” between two of our extinct cousin species. Follow-up studies could crack the mystery — not only for the early humans who lived in the cave complex known as Sima de los Huesos (Spanish for “Pit of Bones”), but for other mysterious populations in the Pleistocene epoch.

“Ancient DNA sequencing techniques have become sensitive enough to warrant further investigation of DNA survival at sites where Middle Pleistocene hominins are found,” the research team, led by Matthias Meyer and Svante Pääbo of Germany’s Max Planck Institute for Evolutionary Anthropology, wrote in Thursday’s issue of the journal Nature. (“Hominin” is the currently accepted term for humans and our close evolutionary cousins.)

As anthropologists are getting better at extracting DNA from ancient bones, genetic mysteries are cropping up more frequently: Last month, researchers at scientific meetings talked about not-yet-published findings that hinted at interbreeding among Neanderthals, Denisovans and previously unknown populations of early humans.

A new standard The age of the mitochondrial DNA analyzed for the Nature study sets a new standard: Researchers used statistical analysis of the DNA and other samples to estimate that the material was roughly 400,000 years old. That meshed with the estimated age for similar DNA extracted from bear bones found in the same cave.

More than 6,000 human fossils, representing about 28 individuals, have been recovered from the Sima de los Huesos site, a hard-to-get-to cave chamber that lies about 100 feet (30 meters) below the surface in northern Spain. The fossils are unusually well-preserved, thanks in part to the undisturbed cave’s constant cool temperature and high humidity.

Image: Femur bone

Javier Trueba / Madrid Scientific Films
The thigh bone of a 400,000-year-old hominin yielded mitochondrial DNA for analysis.

Researchers drilled a series of tiny holes into the cracks in a human femur recovered from the cave to obtain nearly 2 grams (0.07 ounce) of powdered bone. At first, they looked for the signature of ancient nuclear DNA, which could have provided information about the genome of the individual behind the femur — but that information was overwhelmed by the signature of modern-day human contamination.

Then they turned their attention to the mitochondrial DNA, which lies outside the cell’s nucleus and is passed down from a mother to her children. That strategy was more successful.

Unusual finding Previous analysis of bones from the cave had led researchers to assume that the Sima de los Huesos people were closely related to  Neanderthals on the basis of their skeletal features. But the mitochondrial DNA was far more similar to that of the Denisovans, an early human population that was thought to have split off from Neanderthals around 640,000 years ago. The first Denisovan specimens were identified in 2010, based on an analysis of 30,000-year-old bones excavated in Siberia.

Image: Sima skeleton

Javier Trueba / Madrid Scientific Films
This skeleton from the Sima de los Huesos cave has been assigned to an early human species known as Homo heidelbergensis. However, researchers say the skeletal structure is similar to that of Neanderthals – so much so that some say the Sima de los Huesos people were actually Neanderthals rather than representatives of Homo heidelbergensis.

The latest DNA analysis sent scientists scrambling for an explanation.

“This unusual finding could be due to at least two different scenarios, both relating to the material inheritance of mtDNA [mitochondrial DNA] and the ease with which it can be lost in a lineage,” Chris Stringer, a paleoanthropologist at London’s Natural History Museum who was not involved in the Nature study, wrote in an email.

One scenario could be that the DNA was passed down the maternal line from a population that was ancestral to the Sima de los Huesos humans as well as the Denisovans, but that the lineage died out among Neanderthals and modern humans.

The other scenario is that an as-yet-undetermined population interbred with ancestors of the Spanish cave-dwellers as well as the Denisovans. Meyer, Pääbo and their colleagues tentatively favor that scenario. “Based on the fossil record, more than one evolutionary lineage may have existed in Europe during the Middle Pleistocene,” they write.

“Either way, this new finding can help us start to disentangle the relationships of the various human groups known from the last 600,000 years,” Stringer said. “If more mtDNA can be recovered from the Sima ‘population’ of fossils, it may demonstrate how these individuals were related to each other, and how varied their population was.”

Update for 10:30 p.m. ET Dec. 4: Erik Trinkaus, a paleoanthropologist at Washington University in St. Louis, told NBC News that the DNA findings were interesting from a technical standpoint — but he pointed out that the mitochondrial DNA alone doesn’t reveal how the Sima de los Huesos people and their ancestors lived. He suspects that further DNA studies will show that the relationships between populations of early humans were messier and more tangled than the typical diagrams of human origins would suggest. That’s appropriate, he said, “because I think the real world is messy.”

More about our tangled origin story:

In addition to Meyer and Pääbo, authors of “A Mitochondrial Genome Sequence of a Hominin From Sima de los Huesos” include Qiaomei Fu, Ayinuer Aximu-Petri, Isabelle Glocke, Birgit Nickel, Juan-Luis Arsuaga, Ignacio Martínez, Ana Gracia, José María Bermúdez de Castro and Eudald Carbonell.

Alan Boyle is NBCNews.com’s science editor. Connect with the Cosmic Log community by “liking” the NBC News Science Facebook page, following @b0yle on Twitter and adding +Alan Boyle to your Google+ circles. To keep up with NBCNews.com’s stories about science and space, sign up for the Tech & Science newsletter, delivered to your email in-box every weekday. You can also check out “The Case for Pluto,” my book about the controversial dwarf planet and the search for new worlds.

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PatAgonica

Soy el Dr. Gastón Alejandro Saint Martin (ARDMS. RVT. RPVI eligible, MD. CCPM). Medico patagónico; me gradué en la UNBA (Universidad Nacional de Buenos Aires, en 1961) Recién graduado y casado fui a hacer mi entrenamiento en la Universidad de Louisville, (UdeL) Kentucky, USA, la mas antigua Universidad Municipal de La Unión; pobre, pero de excelente nivel académico; con una pequeña escuela de medicina. Louisville está en la margen izquierda del rio Ohio (afluente del Mississippi) Elegí a UdeL por estar entre los rio Ohio y Tennessee, que era la zona mas subdesarrollada de La Unión, (cuna de los "Hillbillies") montañeses pobres, poco ilustrados, casi analfabetos pero gente honrada, noble y muy trabajadora; donde era de esperar encontrar problemas de patología medica y socio económicos similares a los de la población rural patagónica indigente. Los médicos residentes de la Universidad de Louisville atendíamos al Louisville General Hospital y al Children’s Hospital of Louisville. Finalizado mi entrenamiento, y al tener yo visa de emigrante, me pude quedar en USA, donde los abogados jueces y los médicos con residencias de entrenamiento completas, teníamos un brillante futuro económico, estabilidad y brillo social. La tentación para quedarse fue muy grande, pero cumplimos mi plan regresando a casa; Argentina (No a Buenos. Aires.) sino a La Patagonia (Gral. Roca, Rio Negro), donde trabajé intensamente, no solo en medicina asistencial sino en política. Para el inicio, recluté un pequeño grupo de médicos argentinos jóvenes entrenados en USA y Canadá con quienes creamos al Instituto Medico del Comahue, el pequeño hospital austral privado pionero en medicina asistencial moderna. Construimos el edificio por esfuerzo propio, y por administración, con la dirección técnica de mi padre (Ingeniero Prospero Saint Martin). Tuvimos muchos éxitos y fracasos. Nos integramos al Colegio Medico de General Roca, donde fui el primer organizador de la primer biblioteca medica patagónica. De nuestros Colegas Veteranos aprendimos la Filosofía Ético Moral Medica Hipocrática) y a ser médicos humanistas, antes que corporativos." Allí, practicando, aprendimos las normas básicas de cortesía entre colegas y pacientes. No fue fácil la convivencia y el pasaje armonico de lo (clásico) antiguo a lo (bueno) moderno, pero ¡lo logramos ... y muy Bien! En el ano 2000/2002 La Anarquía establecida Argentina desde el 10 de Septiembre de 1930 estalló y ya “no pude tolerar al criminal anárquico mamarracho republicano Argentino" que culminó con el "Robo Legalizado" (Google "The Legalized Theft" by Steve Hanke - CATO Instituto and/or Johns Hopkins economist) Como muchos de los argentinos ordenados, responsables, con importante capital de trabajo sin deudas, con algunos ahorros PERDIMOS TODO! Yo no perdí mis edificios, mi casa, mi Consultorio Radiológico) ni mi costoso instrumental diagnostico pero al quedar fuera de corporaciones medicas-hospitalarias corruptas, y fuera de los canales de facturación y cobro ... me encontré despojado de uno de los derechos humanos mas importantes “EL DERECHO A TRABAJAR” , del que depende mi “DERECHO A LA PROPIEDAD” y derecho a de “VIVIR en LIBERTAD” como me de la gana y sin pedir nada a nadie (¡LIBRE ALBEDRIO!) Por ello decidimos volver (ya estando jubilados) por segunda vez a USA. Hoy tengo otra residencia legal en Chicago (Oak Park, 60301) Mi mentor (en USA y Argentina) ha sido el Dr. Rene Favaloro (pionero del bypass coronario), con quien (cada uno por su lado) hemos compartido los mismos dilemas, para decidir volver, y para adaptarnos a esta ANARQUÍA Argentina (ver www.29deJulio.wordpress.com ) – La ANARQUÍA en la que cayó La Republica Argentina el 10 de Septiembre de 1930, fue causado por errores garrafales del GOBIERNO DE LOS ESTADOS UNIDOS DE NORTEAMÉRICA. (Presidente Herbert Hoover (POTUS 31) ...MAS... errores garrafales en la "ACORDADA UNÁNIME de LA TOTALIDAD DE LOS JUECES DE LA CORTE DE JUSTICIA ARGENTINA" ...MAS... errores garrafales del Gobierno del Reino Unido de Gran Bretaña (UK) ...MAS... el error de un soldadito con grado de General y cáncer de estomago Gral. Félix Uriburo) que no se atrevio a desobedecer al Jefe de su jefe cuando le ordenaron "violar a La Constitucio ... "en nombre de ella??) y asi TODOS ELLOS JUNTOS, en un contubernio internacional, por primera vez en la historia de La Republica Argentina depusieron a un Presidente Constitucional, (Presidente Hipólito Yrigoyen) electo dos veces (en periodos NO consecutivos), por el voto popular, y depuesto con la absurda e ignorante excusa (si no fue mentirosa) de ser "ser viejo e inoperante..."

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